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The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatants 48 h later。

FeFAB cells (a kind gift from Dr Martin Löchelt, Germany) are modified CRFK cells that are used for FFV infection experiments. FeFAB cells express β-galactosidase under the control of the FFV LTR, which is trans。

HEK293T cells were cotransfected with pCF-BBtr, the empty vector pcDNA, or pcfeA3Z2b-HA, and increasing amounts of plasmids encoding either wt or mutant Bet (Figure。

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